台大醫學中心,過去數十年來,由於研究需要醫院各部門已陸續收集各式正常與不正常、新鮮的或福馬林固定的組織標本。但這些組織分散在醫院各部門或各實驗室一直缺乏人力和物力來整理這些重要研究資源,並無法發揮組織庫的功能,實在非常可惜。
本計畫在台大醫學中心成立,可以匯集台灣各式人類常見與罕見的疾病。我們希望有計畫系統性的收集台大醫院手術取得的各項組織檢體,提供本計畫下同仁研究使用。
未來服務亦可擴及全國之研究單位和生物科技公司。甚而可與世界性之大學組織庫互相作交流溝通,促進基礎生命科學、醫學研究和生物科技產業發展。
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中文摘要

人類基因圖譜粗略完成,有關新基因的發現(或已發現但不知功能的),常需以各種不同器官正常及不正常的組織去做定性與定量分析,以確定此基因分子之正常組織分佈或異常組織表現,進而探索其可能的生物功能與其它可能相關因子之複雜作用。如新穎基因功能可能涉及腫瘤生成,探索其在何種腫瘤生成扮演何種角色,皆需要系統性的檢驗其在各型正常組織、良性或惡性腫瘤的表現,來初窺此基因可能涉及細胞生長或繁殖、細胞凋零、細胞老化、細胞分化、去氧核糖核酸突變修復、或染色體功能等,進而釐定我們研究方向。組織的多樣性、數量性、罕見性及質地上的正確性、優良性,是我們研究的重要基石,我們希望有系統的建立規模完整之組織庫,提供本計畫下同仁研究使用,亦可擴及全國之研究單位和生物科技公司。甚而可與世界性之大學組織庫互相作交流溝通,促進基礎生命科學、醫學研究和生物科技產業發展。


Abstract
Many laboratories in Taiwan have isolated and identified some novel genes. But they always suffer from shortage of tumor and their surrounding normal tissues for characterization of those novel genes. Besides, the rapid progress genome project leads to the discovery of vast amount of coding and non-coding DNA sequences, which still need much effort for their biological functional assignment. In the three years, although NSC and NHRI have established four tumor tissue banks located at four different hospital at Taiwan, the scale was limited to tumor sample collection only and the fund was thus restricted so that it was almost impossible establish tissue banks with varying kinds of human tissue samples to cover as much as possible kinds of human diseases by this way. Thus, it is important to systemically establish the tissue bank with excellent quality and quantity in Taiwan, in order to provide materials for in depth research in the post-genomic era. National Taiwan University Hospital (NTUH) as a national medical center is the place where rare or complicated medical diseases come for an accurate diagnosis and treatment. In cooperation with excellent surgeons and pathologists, we are in a good position to collect human tissues with excellent quality and accurate diagnosis, which will be valuable for researches. These materials can be easily collected to set up a tissue bank. These materials with well documented diagnosis, either in fresh frozen state or embedded in paraffin blocks, can be used to perform immunohistochemistry, immunofluorescent localization, in situ PCR or RNA/DNA in situ hybridization. Furthermore, this well organized tissue bank can use the tissue to prepare a DNA/RNA/cDNA and Western blotting to characterize the mRNA expression or gene dosage from each novel gene cloned from different cancer tissues. In addition, DNA/RNA/protein from different types of tumor cells can be used to characterize the behavior of a given molecule in tumorigenesis. We also collect benign tumor tissues and normal/abnormal embryos and tissues and blood of other rare diseases.





目前可提供之服務有免疫染色、tissue array (low dendity), 雷射細胞擷取(LCM),各研究人員如有需求可恰服務人員。

組織之種類如表列,如需詳情可洽本計畫之服務人員

1. 提出申請時,請送交申請書一份及研究計畫簡述三份。
2. 為使用組織庫之資源能分享給更多使用者,希望每次申請數量以 (a) DNA:30例 (b) RNA:30例 (c) 液態氮冷凍標本:30例 (d) OCT包埋切片標本:30例 (e)免疫組織染色:50例 為限。

瞭解相關作業依需要提出申請,首先分別下載免疫染色申請表格,將資料經由 FAX,e-mail 或逕行送交組織庫核心實驗室。收件後申請資料將送交組織庫核心實驗室使用者委員會核定。依收件順序,審核時間約一週,並告知審核結果。申請注意事項:

審核通過者,將通知實驗安排或標本領取時間。請注意下列事項:

a. 申請免疫組織染色者,請附上足量之 primary antibody 及 protocal。若需切片者,請附上玻片及標本請先浸泡福馬林,再放入粉紅色標本匣內,送標本前天請用流動清水浸泡至當天,再將標本匣撈起放入夾鏈袋,標上送件單位、聯絡人、及連絡電話。
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意見及問題歡迎來電或寫信至 tissuebank2011@gmail.com
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